Fluorescence Ihuza Iyungurura
Incamake y'ibicuruzwa
Akayunguruzo ka Fluorescence nikintu cyingenzi gikoreshwa mubikoresho byubuzima nubuzima.Igikorwa cyacyo nyamukuru ni ugutandukanya no guhitamo ibiranga uburebure bwumurongo wumucyo ushimishije hamwe na fluorescence yibintu muri sisitemu yo kugenzura no gusesengura biomedical fluorescence.Akayunguruzo ka Fluorescence karangwa nuburebure bwimbitse-buke na autofluorescence.Mubisanzwe, muyunguruzi nyinshi irashobora gufatanyirizwa hamwe kugirango ikore fluorescence iyungurura, ifite imikorere ihenze cyane.
Umwanya wo gusaba
Akayunguruzo ka Fluorescence gakoreshwa mugihe nyacyo fluorescence igereranya ibikoresho bya PCR.ikoreshwa cyane muri biologiya ya biologiya no kumenya umutekano w’ibiribwa no gukurikirana icyorezo cy’ubuzima rusange n’izindi nganda.
Akayunguruzo ka Fluorescence gakoreshwa mugutahura amarangi atandukanye hamwe nubushakashatsi ， urugero:
FAM / SYBR Icyatsi / Icyatsi / HEX / TET / Cy3 / JOE / ROX / Cy3.5 / Texas Umutuku, Cy5 / LC Red640, Cy5.5 nibindi
| Inzira | (IAD Ikomeye) | ||
| Uburebure | Ex （nm） | Em (nm) | Umusaraba |
|
| 470-30 | 525-20 | > 6 |
|
| 523-20 | 564-20 | > 6 |
|
| 543-20 | 584-20 | > 6 |
|
| 571-20 | 612-20 | > 6 |
|
| 628-35 | 692-45 | > 6 |
| Guhagarika | OD> 6 @ 200 ～ 900nm cyangwa @ 200 ～ 1200nm | ||
| Umusozi (nm) | 50% ~ OD5 <10nm | ||
| Umusaraba | OD> 6 | ||
| Ingano (mm) | Φ4mm, Φ12mm, Φ 12.7mm, Φ25.4mm n'ibindi | ||
Ikirangantego
Uburyo bwo gukora
